Watch Cooper's full interview with Conway below (the eye roll occurs just after the 10-minute mark). "Could you imagine rolling your eyes, having a male anchor on a network roll eyes at Hillary Clinton ... a female representative/spokesperson for President Obama or President Bill Clinton?".
But Conway said she thought people would have reacted differently if she wasn't the target.
Conway, like President Trump, cited the FBI director's controversial handling of the investigation into Hillary Clinton's use of a private email server as the reason for his termination. "You're conflating two things that don't belong together", she said.
"Thanks for the trip down memory lane". I said, "thanks for the trip down memory lane" and talked about winning MI and then he rolled his eyes.
Ms Conway later claimed: "This has nothing to do with the campaign from six months ago, this has everything to do with the performance of the Federal Bureau of Investigation director since the President has been in the White House".
Her absence has been so notable that "Saturday Night Live" addressed the matter over the weekend, in a skit about a children's game show called "Where in the World is Kellyanne Conway?".
West Brom boss Tony Pulis hoping to delay Chelsea title celebrations
In the second half also, Chelsea dominated the game and did not give any chance to Middlesbrough to make any threatening move. I haven't played every single game but I feel the quality of my minutes this season has been fantastic.
Fireworks cache explodes in Mexico, killing at least 12
Saints' days, Christmas and New Year parties in many Latin American countries often wrap up with a fireworks free-for-all. The fireworks explosion in San Isidro, a village 170 miles east of Mexico City, is the second incident in recent months.
Pak violates truce agreement on LoC in Rajouri, Poonch
In the video footage it was clearly visible that anti-tank guided missiles were used to destroy Pakistani bunkers along the LoC. The video could have been shot just a few days ahead of the beheading of two Indian jawans by Pakitani security forces.
So, was Cooper's eye-roll sexist? In January, she appeared on his show and questioned the validity of a CNN report claiming that top intelligence officials had presented Trump (then a President-elect) with a list of allegations that Russian operatives may be in possession of compromising personal and financial information about him.
Conway last month attempted to redefine feminism - and the dictionary really wasn't impressed.
"Let me tell you something". Her refusal to acknowledge universal sexism does not negate the fact that she can experience it herself, but she should have acknowledged that Clinton and other women have also had similar experiences rather than trying to distance herself from them.
While making no mention of the emails in a letter to Comey, Trump did explain that the dismissal was to restore "public trust and confidence".
"You know you want to question the timing of when he fires, when he hires", she said.
Both statements were contradicted shortly afterwards by White House spokesman Sean Spicer.